Uristix  Glucose  &  Protein  Test  Strips

Semi quantitative test for glucose and protein. Pack of 100 Uristix strips incorporating reagent areas that test specifically for glucose and protein, and change colour if either is present in solution. 

Testing is carried out at room temperature and the colour of the reagent areas are compared to the colour chart on the bottle label.  Check for the presence of glucose 30 seconds after wetting.  Check for the presence of protein 60 seconds after wetting.

Glucose results are obtained directly from comparison to the colour chart 30 seconds after wetting the test strip.  Each colour block represents a range of values.  The colour blocks represent nominal values; actual values will vary around the nominal values.  Results are read from the colour chart as negative or varying degrees of positive which indicate the relative amounts of glucose present.  Colour blocks are designated as:

Negative,  5.5mmol/L (100mg/dL),  14mmol/L (250mg/dL),  28mmol/L (500mg/dL),  56mmol/L (1000mg/dL),  and  111mmol/L (2000mg/dL) or more.

Protein results are obtained directly from comparison to the colour chart 60 seconds after wetting the strip.  Each colour block represents a range of values.  The colour blocks represent nominal values; actual values will vary around the nominal values.  Results are read from the colour chart as negative or varying degrees of positive which indicate the relative amounts of protein present.  Colour blocks are designated as:

Negative,  Trace,  0.30g/L,  1g/L,  3g/L,  and  20g/L or more.

Note:  The protein reagent system is more sensitive to albumin than to globulins, haemoglobin, Bence-Jones protein and mucoprotein; a negative result does not rule out the presence of these other proteins.

Chemical Principles of Procedure

The glucose test is based on a double sequential enzyme reaction.  One enzyme, glucose oxidase, catalyzes the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose.  A second enzyme, peroxidase, catalyzes the reaction of hydrogen peroxide with a potassium iodide chromogen to oxidise the chromogen to colours ranging from green to brown.

The protein test is based on the protein-error-of-indicators principle.  At a constant pH, the development of any green colour is due to the presence of protein.  Colours range from yellow for "Negative" through yellow-green and green to green-blue for "Positive" reactions.